Clinical Trial: Studying the Relationship Between Contact Lens Packaging Solutions and Bacterial Binding to Surface Eye Cells

Study Status: Completed
Recruit Status: Completed
Study Type: Interventional

Official Title: A Masked, Randomized, Prospective, Single Center Crossover Clinical Trial on the Relationship Between Chemically Preserved Contact Lens Packaging Solutions and Bacterial Binding to Corneal Epithelial

Brief Summary: The goal of this study is to investigate the overall theory that the use of chemically preserved solutions associated with contact lenses promotes an increase in bacterial infection.

Detailed Summary: The purpose of this study is to investigate the theory of bacterial binding to surface eye cells through the use of chemically preserved solutions.
Sponsor: University of Texas Southwestern Medical Center

Current Primary Outcome: Bacterial binding of Pseudomonas Aeruginosa [ Time Frame: 5 weeks ]

Counting the number of bacteria that bind to surface epithelial cells.


Original Primary Outcome: Bacterial binding of Pseudomonas Aeruginosa [ Time Frame: 3 weeks ]

Corneal irrigant is mixed with patient samples for 30 min, then stopped for 5 min. Cell suspension is passed through a filter holder to collect cells. Each filter is washed and stained, three times. The membrane filter is placed on a microscope slide to air dry, and heated for 5 min to immobilize bacteria. Filter membrane is cooled and restained. A Leica fluorescent microscope with an epifluorescence attachment is used to examine PA biding to epithelial cells under oil immersion. The total number and size of corneal epithelial cells on filter are counted and recorded.


Current Secondary Outcome:

Original Secondary Outcome:

Information By: University of Texas Southwestern Medical Center

Dates:
Date Received: April 9, 2010
Date Started: April 2010
Date Completion:
Last Updated: October 13, 2010
Last Verified: April 2010