Clinical Trial: Abnormal Expression Proteins, Mitochondrial DNA and miRNA of Irritable Bowel Syndrome

Study Status: Recruiting
Recruit Status: Unknown status
Study Type: Observational

Official Title: Screening and Identification of Abnormal Expression Proteins and miRNA From Colonic Mucosa of Patients With Irritable Bowel Syndrome.

Brief Summary: In the investigators study, the investigators will focus on the screening of the related proteins and miRNA to IBS in order to reveal the possible clues or molecular mechanism for this disorder.

Detailed Summary: For the proteomic procedures, Our study groups extracted proteins from the cecum and sigmoid colon samples and ran on the two-dimensional gel electrophoresis (2-DE). The proteins on 2-DE were visualized by the silver stain, and then gel maps were scanned and analyzed with the Image Master 2D Elite. The obvious abnormal protein spots were identified by the mass spectrometry. Western blot 、 immunohistochemistry 、 mRNA and miRNA were also performed for further verification of the proteins.
Sponsor: Chinese PLA General Hospital

Current Primary Outcome:

Original Primary Outcome:

Current Secondary Outcome:

Original Secondary Outcome:

Information By: Chinese PLA General Hospital

Dates:
Date Received: December 8, 2009
Date Started: January 2006
Date Completion: December 2012
Last Updated: February 10, 2011
Last Verified: December 2006